During a engagement to Lichfield Cathedral on Monday, King Charles was shouted at by a protester who asked him about his brother's connections with disgraced financier Jeffrey Epstein.
The individual inquired how long the King had been known about his sibling's relationship with Epstein, and also questioned whether he had asked the authorities to "hide" for Andrew.
Amid the protest, there were large groups of well-wishers and applause for the King, who was in the region for an engagement.
The Duke of York's connections with Jeffrey Epstein have been under intense scrutiny in recent weeks. The prince, who relinquished his titles earlier this month, has repeatedly denied any misconduct.
"For how long have you been aware about Andrew and Epstein? Did you request the police to conceal for Andrew?"
The monarch ignored the questions but carried on meeting supporters, some of whom shouted "Long live the King". One fan can be heard asking him for a embrace.
A female bystander outside the cathedral was also observed telling the heckler to "leave" and to "be quiet".
The controversy over the Duke of York's connections to Epstein has been reignited by misconduct claims repeated in Virginia Giuffre's posthumously-published book.
In the publication, the accuser alleges she had sex with the prince on three separate occasions, including one instance with Epstein and "several other minors"
Andrew, who reached a financial settlement with the plaintiff in 2022, has consistently denied any wrongdoing. In 2019, he told a news program he did not recall meeting her "ever", and that they "never had any sort of sexual contact."
But in recent times, new inquiries have been raised about how he is able to finance his way of life despite not being a active member of the royal family.
The revelation that he has only ever paid a nominal fee on his Royal Lodge has intensified the scrutiny over his housing situation - even though he made significant contributions up front, including for renovations.
The Liberal Democrats said he should be called in before MPs to give evidence about his lease of his residence.
But last week, the government refused to give MPs time to discuss the royal's titles or his home, despite growing pressure for scrutiny.
Buckingham Palace has not issued a statement on whether Prince Andrew might move out of his home - or where he might reside.
A seasoned business strategist with over 15 years of experience in digital innovation and corporate consulting across various industries.